10.6084/m9.figshare.2412802.v1 Andrés Egea-Serrano Andrés Egea-Serrano Josh Van Buskirk Josh Van Buskirk Responses to nitrate pollution, warming and density in common frog tadpoles (Rana temporaria) Brill Online 2016 density fertilizer natural stressors pollution Rana temporaria warming Zoology Ecology 2016-02-19 08:22:56 Dataset https://brill.figshare.com/articles/dataset/Responses_to_nitrate_pollution_warming_and_density_in_common_frog_tadpoles_Rana_temporaria_/2412802 Amphibians face a variety of anthropogenic environmental perturbations that could act alone or in combination to influence population size. We investigated interactive effects of warming conditions, a moderate pulse of nitrogen pollution, and conspecific density on larvae of the common frog, <i>Rana temporaria</i>. The 16-day experiment had a 2 × 2 × 2 factorial design implemented in 80-l Ecology outdoor mesocosms. High density and warm temperature both resulted in reduced activity and visibility; tadpoles grew and developed more quickly at low density and high temperature. The high-nitrogen treatment did not influence behavior, growth, or development rate. We attribute this to several realistic features of our study, including a pulsed treatment application and natural denitrification within the mesocosms. There was only a single interaction among the three factors: higher temperature exacerbated density-dependence in growth rate. These results illustrate that climate warming may benefit temperate amphibians, although the benefits may be counteracted by enhanced larval crowding.