The complete plastid genome sequence and the photosynthetic activity of the putative mycoheterotrophic orchid Limodorum abortivum: Supplementary material
Sofia Shevtsov
Omer Murik
Hagit Zer
Ofir Weinstein
Nir Keren
Ori Fragman-Sapir
Oren Ostersetzer-Biran
10.6084/m9.figshare.7228451.v1
https://brill.figshare.com/articles/figure/The_complete_plastid_genome_sequence_and_the_photosynthetic_activity_of_the_putative_mycoheterotrophic_orchid_Limodorum_abortivum_Supplementary_material/7228451
<p>The sparsely
distributed <i>Limodorum abortivum</i> is a European-Mediterranean orchid
species, which grows on decomposing plant material. Although some chlorophyll-pigmentation
is observed in the degenerated scales-shaped leaf and stems regions of the
plant, its photosynthetic capacity is assumed to be insufficient to support the
full energy requirements of an adult plant. In Israel, <i>L. abortivum</i> shows
a patchy distribution patterns in the Galilee, Golan, Carmel and Judean
regions. To gain more insights into the physiology and photosynthetic activity
of <i>L. abortivum</i>, we analyzed the organellar morphologies, photosynthetic
activities the chloroplast-DNA sequence by Illumina-HTS. Microscopic analyses
indicated to the presence of mature chloroplasts with well-organized grana-thylakoids
in the leaves and stems of <i>L. abortivum</i>. However, the numbers of chloroplasts
per cell and the grana ultrastructure density within the organelles were notably
lower than those of model plant species and fully photosynthetically-active
orchids. The cpDNA of <i>L. abortivum</i> (154,954 bp) encodes 60 proteins, 34
tRNAs and 4 rRNAs. The coding-regions of 24 genes are interrupted by 26 group-II
intron-sequences. While many genes related to photosynthesis (RuBisCo, PSI,
PSII and cytochrome <i>b<sub>6</sub>/f</i> subunits) have remained intact in
the cpDNA, the majority of the NADH-dehydrogenase (<i>ndh</i>) subunits were
either lost or became nonfunctional (i.e. pseudogenized). In agreement with
previous reports, the photosynthetic-rates of adult <i>Limodorum</i> plants were
found to be very low, further indicating that carbon-assimilation activity is
insufficient to support the energy requirements of an adult plant, and may
suggest that <i>L. abortivum</i> have adopted nutritional strategies similar to
that of mycoheterotrophic orchid species. </p>
2018-12-05 09:09:04
Chloroplast
cpDNA
photosynthesis
Limodorum
orchids
mycoheterotrophic
plants
Plant Biology not elsewhere classified