10.6084/m9.figshare.9009626.v1
Li Gong
Li
Gong
Xiaoyu Kong
Xiaoyu
Kong
Hairong Luo
Hairong
Luo
Shixi Chen
Shixi
Chen
Wei Shi
Wei
Shi
Min Yang
Min
Yang
Intra-genomic variability and pseudogenes in ribosomal ITS regions of Paraplagusia blochii (Pleuronectiformes: Cynoglossidae): supplementary material
Brill Online
2019
Ribosomal RNA
secondary structure
Bloch’s tonguesole
flatfish
non-concerted evolution
Ecology not elsewhere classified
2019-09-16 11:41:36
Journal contribution
https://brill.figshare.com/articles/journal_contribution/Intra-genomic_variability_and_pseudogenes_in_ribosomal_ITS_regions_of_Paraplagusia_blochii_Pleuronectiformes_Cynoglossidae_supplementary_material/9009626
Eukaryotic nuclear ribosomal DNA (<a></a><a>rDNA</a>)
typically evolves in a concerted manner, in which hundreds of rDNA sequences
within species show little or no variations, whereas the sequences of different
species diverge. There are few studies of the internal transcribed spacer
regions (ITS1–5.8S–ITS2) in teleostean fishes and only one report on
flatfishes. Here, we reported the discovery of two types of highly divergent
ITS1–5.8S–ITS2 rDNA sequences (Type A and B) in the genome of the Bloch’s
tonguesole, <i>Paraplagusia blochii</i>.
These sequences mainly differ in sequence length, secondary structure, and
minimum free energy. According to the potential features of pseudogenes, Type B
sequences are speculated to be putative <a></a><a></a><a></a><a>pseudogen</a>ic ITS
regions. Cluster analyses also supported two major clades that corresponded to
the sequence type. To the best of our knowledge, this is the first report of
the ITS regions of tonguefish, and may therefore provide useful information for
future studies of the rDNA of flatfishes as well as the patterns of rDNA
evolution in teleostean fishes