Supplementary materials to In vitro incubation reveals the human overall gut microbiota composition is resilient to changes in methanogenesis (10.1163/18762891-bja00059)

TABLE S1 Characteristics of the participants included in this study.

FIGURE S1 Relative abundance of genus Methanobrevibacter, throughout the incubation with pectin or the carbohydrate mixture in methane excretors (A) and non-methane excretors (B). Lines represent the average of the duplicate incubations at each timepoint. Mixture: carbohydrate mixture consisting of equal amounts of apple pectin, potato starch, inulin, beechwood xylan, and arabinogalactan. BES: 2-bromoethanesulfonate. M, methane excretor; N, non-methane excretor.

FIGURE S2 Overview of the sulphate concentrations measured during the different incubations. Mixture, carbohydrate mixture consisting of equal amounts of apple pectin, potato starch, inulin, beechwood xylan and arabinogalactan; M, methane excretor; N, non-methane excretor.

FIGURE S3: Relative abundance of genus Desulfovibro throughout the incubation with pectin or the carbohydrate mixture in methane excretors (A) and non-methane excretors (B). Lines represent the average of the duplicate incubations at each timepoint. Mixture, carbohydrate mixture consisting of equal amounts of apple pectin, potato starch, inulin, beechwood xylan and arabinogalactan. M, methane excretor; N, non-methane excretor.

FIGURE S4 Adding BES in non-methane excretors did not change overall microbiota composition. PCoA analysis based on Bray-Curtis dissimilarity using the faecal slurries of non-methane excretors in the incubations with pectin and the carbohydrate mixture (A). Top 10 family-level taxa ranked based on the average relative abundance across all the incubation samples using the faecal slurries of non-methane excretors with pectin and the carbohydrate mixture (B). Mixture, carbohydrate mixture consisting of equal amounts of apple pectin, potato starch, inulin, beechwood xylan and arabinogalactan; N, non-methane excretor; BES, 2-bromoethanesulfonate.

FIGURE S5 The addition of sulphate did not change overall microbiota composition. PCoA analysis based on Bray-Curtis dissimilarity using the faecal slurries of methane excretors in the incubations with pectin and the carbohydrate mixture (A) and of non-methane excretors in the incubations with pectin and the carbohydrate mixture (B). Top 10 family-level taxa ranked based on the average relative abundance across all the incubation samples using the faecal slurries of methane excretors with pectin and the carbohydrate mixture (C) and of non-methane excretors in the incubations with pectin and the carbohydrate mixture (D). Mixture, carbohydrate mixture consisting of equal amounts of apple pectin, potato starch, inulin, beechwood xylan and arabinogalactan; M, methane excretor; N, non-methane excretor.