Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain: supplementary material

<p>The genus <i>Longidorus </i>constitutes a large group of approximately 170 species of plant-ectoparasitic nematodes that are polyphagous and distributed almost worldwide. Some of the species of this genus are vectors of plant viruses. Species discrimination in <i>Longidorus </i>is difficult because the morphology is very conservative, and morphometric characters often overlap, leading to potential misidentification. Integrative taxonomy, based on the combination of molecular analyses with morphology, is a useful and necessary approach in <i>Longidorus </i>species identification. In Spain from 2014 to 2017, we conducted nematode surveys among cultivated and wild plants, from which we identified 13 populations of <i>Longidorus</i>, two of which appeared to represent new species and are described herein as <i>L. iliturgiensis </i>sp. nov. and <i>L. pacensis </i>sp. nov., and 11 populations belonging to eight known species: <i>L. africanus</i>, <i>L. baeticus</i>, <i>L. carpetanensis</i>, <i>L. fasciatus</i>, <i>L. nevesi</i>, <i>L. cf. olegi</i>, <i>L. pini</i>, and <i>L. vallensis</i>. Three species are new geographical records for Spain (<i>L. nevesi</i>, <i>L. cf. olegi</i>, and <i>L. africanus</i>). We report molecular data for <i>L. nevesi</i>, <i>L. cf. olegi</i>, <i>L. carpetanensis</i> and <i>L. pini </i>for the first time. Additionally, we describe the males of <i>L. pini</i> and the juveniles of <i>L. cf. olegi</i>.</p>