Suppl. material to: The phylogeny of the tribe Podonomini (Chironomidae: Podonominae) is clarified by new inclusion of <i>Rheochlus</i> Brundin in molecular analysis

<p dir="ltr">The immature stages of the patagonian podonomine (Diptera: Chironomidae) genus Rheochlus (Diptera:</p><p dir="ltr">Chironomidae) have been unknown although the adult stage was described over half a century ago. New</p><p dir="ltr">pupal associations with described adults belonging to two species of Rheochlus allow better understanding</p><p dir="ltr">of the genus, its diversity and its phylogeny. The pupa of Rheochlus insignis Brundin is the same</p><p dir="ltr">as an unreared damaged pupal taxon designated Podochlus sp. “Canteras’‘ by Brundin and is described</p><p dir="ltr">more completely here. Inclusion of R. insignis in a molecular phylogenetic analysis shows it to be sister</p><p dir="ltr">to two specimens (one a male, the other pharate female) allocated previously to Podochlus but better</p><p dir="ltr">transferred to Rheochlus. The male morphology is compatible with Rheochlus prolongatus Brundin but we</p><p dir="ltr">refrain from asserting identity by using ‘cfr.’ prolongatus to indicate the status. Molecular evidence also</p><p dir="ltr">provides association of the female adult and pupa that are newly described here. We validate Rheochlus</p><p dir="ltr">as distinguished on pupa and adult, with the type-species Rheochlus insignis from Patagonian Chile and</p><p dir="ltr">Argentina, Rheochlus prolongatus Brundin from Southern Patagonia Argentina and possibly also from</p><p dir="ltr">central Patagonia, Rheochlus latisetus Siri & Brodin from Patagonia, and the eastern Australian Rheochlus</p><p dir="ltr">wirthi Brundin. The latter two species remain known only from their original adult male descriptions.</p><p dir="ltr">Strengthened support for our concept of Rheochlus as a valid genus, sister group to Podochlus, derives also</p><p dir="ltr">from an expanded and reanalysed morphological matrix.</p>