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Supplementary files for Beneficial Microbes: Butyrate-producing bacteria as probiotic supplement: beneficial effects on metabolism and modulation of behaviour in an obesity mouse model

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posted on 2024-09-10, 10:31 authored by A.M. Garcia-Serrano, C. Skoug, U. Axling, E.R. Korhonen, C. Teixeira, I.L. Ahrén, I. Mukhopadhya, N. Boteva, J. Martin, K. Scott, S. Gratz, K.G. Stenkula, C. Holm, J.M.N. Duarte

Obesity is a risk factor for cardio-metabolic and neurological disease. The contribution of gut microbiota to derangements of the gut-brain axis in the context of obesity has been acknowledged, particularly through physiology modulation by short-chain fatty acids (SCFAs). Thus, probiotic interventions and administration of SCFAs have been employed with the purpose of alleviating symptoms in both metabolic and neurological disease. We investigated the effects of four butyrate-producing bacteria from the Lachnospiraceae family on the development of metabolic syndrome and behavioural alterations in a mouse model of diet-induced obesity. Male mice were fed either a high-fat diet (HFD) or an ingredient-matched control diet for 2 months, and bacteria cultures or culture medium were given by gavage to HFD-fed mice every second day. Mice were assessed through a battery of metabolic and behaviour tests, and fluxes through the gut barrier and blood-brain barrier were determined using Dextran-based tracers. One of the administered bacteria from the Coprococcus genus, which produces butyrate and formate, afforded some degree of protection against the development of obesity and its complications. Results from this study, however, are insufficient to support brain health benefits of the bacteria tested. None of the bacteria modulated permeability through the gut or blood-brain barriers. Our results suggest health benefits of a bacteria from Lachnospiraceae family, and encourage further exploration of its use as probiotic.

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